The natural substrate of the eukaryotic replisome is chromatin and not just DNA. During replication, parental histones must be disassembled ahead of the replication fork and redeposited onto duplicated DNA. We plan on imaging reconstituted chromatin replication reaction to understand how parental histones are redistributed at the replication fork. To achieve this goal we will first need to characterise DNA replication origin activation under the electron microscope.
Miller TC, Costa A. THE ARCHITECTURE AND FUNCTION OF THE CHROMATIN REPLICATION MACHINERY. Curr Opin Struct Biol. 2017 Apr 15;47:9-16.
Wilson MD*, Costa A*. CRYO-ELECTRON MICROSCOPY OF CHROMATIN BIOLOGY. (*Corresponding authors). Acta cryst D. 2017 April 20; ePUB.