We aspire to perform biochemical experiments under the cryo-electron microscope. The system we use is highly complex, involving sequential ATP hydrolysis, post-translational modification and nucleotide switch and incorporation events. We are devising new biochemical tools to minimise compositional heterogeneity in our sample, as well as computational methods to reconstitute replication reactions in silico.
Goswami P, Locke J, Costa A. PREPARING FROZEN-HYDRATED PROTEIN-NUCLEIC ACID ASSEMBLIES FOR HIGH-RESOLUTION CRYO-EM IMAGING. Book chapter in “Nanoscale Imaging: Methods and Protocols”. The Methods in Molecular Biology series (Springer). Guest editor Yuri Lyubchenko. In press.